•  
Support crystalwind.ca with your donation and help spread spirituality and positivity. Blessings!

This article was posted by CrystalWind.ca

A+ A A-

Choosing the Right ELISA Kit for Your Lab: 5 Crucial Things to Keep in Mind

Choosing the Right ELISA Kit for Your Lab: 5 Crucial Things to Keep in Mind

As the number of infectious diseases in the world grows, there is a need to carry out tests and take preventive measures to stay healthy and protect your loved ones. As doctors and medical researchers, you would want to test the patient for specific antibodies to determine whether they have transmissible infections.

Infectious diseases are a critical matter in medicine, and some of them have no cure. There is, therefore, the need to ensure that all kits at your laboratory are in their perfect condition to provide accurate results. Below, we’ll explore five ways to help you determine the kit you will need.

1. Your Target

Before you buy the kit you need for the ELISA test at hand, you need to know the nature of the cytokine you want to monitor. The characteristics you should watch out for are both biological and chemical. Both of these attributes determine the outcome of your results.

You will, therefore, want to find a kit that has the appropriate buffers that tally with the molecule’s chemistry. Biological aspects also play a major role in what buffers you will need. For instance, the amount of cytokine present in the sample you are going to test can mean that the kit you’ll need may be common or rare. The reason for this is that if, for example, there is very little concentration of cytokine, you’ll need an ELISA kit with high capture sensitivity.

Choosing the Right ELISA Kit for Your Lab: 5 Crucial Things to Keep in Mind

2. ELISA Quality Control

The ELISA market keeps expanding, and the kits you come across may overwhelm you with their variation in design and performance. However, the most important factor would be whether or not you will achieve your desired results. However, this may not come easy.

Quality control ensures that each kit meets the standard industry results after benchmarking by the manufacturer. Reproducibility and recovery are two of the main factors to check when determining the quality of the kit you are buying. You should go for a kit that provides as close to 100% recovery as possible.

3. Incubation

Every ELISA test takes some time before you can read the results. But the time you have to wait varies from one experiment to another. This time can also depend on the type of kit you chose for your lab tests. Incubation usually depends on the samples and temperature, among other factors.

Typically, you want to look at the time it takes for you to get your results, say at room temperature. You will discover that a change in temperature range can lead to a deviation in the timespan you have to wait for the test. Sometimes, setting the temperatures too high or low from the threshold may hinder your kits from functioning at all.

Therefore, while you are expecting to achieve the results in the minimum time possible, you should monitor the temperature at which you are conducting your tests.

Choosing the Right ELISA Kit for Your Lab: 5 Crucial Things to Keep in Mind

4. Type of ELISA

ELISA kits come in various assay formats. Your kit choice depends on what you want to determine from the sample you collect. You can choose your kit from competitive, sandwich, direct, and indirect categories. Each of these assay formats has a unique method and application.

For instance, you would want to choose the direct ELISA for antigen presence detection tests. It becomes relevant because all you need is the identification and in the shortest time possible. There will be no need for recording details and consistent monitoring. Competitive ELISA will help in cases where the antigen concentration is too low for typical detection.

5. The specificity of the ELISA

Nobody wants to perform a test and get the wrong results. That would cause frustration, not to mention a waste of kit and time. Without an accurate choice, your kit may end up reading the wrong results with cross-reactions.

Specificity is necessary for accurate readings. It is the factor that will prevent the antibodies from attaching themselves to the wrong proteins or other components of the kit or medium while carrying out the test. Sometimes, the matrix components alter how antibodies react with antigens, giving you false results. That explains why you need to be keen when picking your ELISA to ensure that they have little to no cross-reactions.

Summary

While ELISA helps to determine the presence of antibodies in blood to confirm the infection or level of the body’s self-defense, inappropriate tests can lead to futile results. That explains why it is necessary to invest your time when choosing the kits for your laboratory. You, therefore, need an understanding of what you are studying before buying the kits for your assessments.

Credit

BIO:

Rebecca Smith is a writer for numerous health and fitness publications. She has been working with many challenged clients for some time now. She can be reached via email at .

This article was submitted exclusively to CrystalWind.ca by Rebecca Smith 

© 2020 crystalwind.ca. All rights reserved. Do Not Copy.

Pin It

Join the Conversation Now! Comment Below! arrow down small 11

 
CrystalWind.ca is free to use because of donations from people like you. Please help support us! 
Blessings!

Follow this blog

Disclaimer Health

All post and information provided within this blog is for educational and informational purposes only, and is not to be construed as medical advice or instruction. No action should be taken solely on the contents of this website.  Please consult with your healthcare professional before making any dietary or lifestyle changes or taking supplements that may interfere with medications. Any products or information discussed are not intended to diagnose, prevent, treat or cure any illness, disease or lifestyle. Please consult your physician or a qualified health professional on any matters regarding your health and wellbeing or on any opinions expressed within this website.

 

Featured Writers

crystal-wind-oracle-mobile-app
Cut Through The Illusions!
Available On
Apple  | Android | Amazon
NEW Expanded Version - 53 cards!

Spirit Animal Totem Of The Day!

CrystalWind.ca is free to use because of
donations from people like you.
Donate Now »

CrystalWind.ca Donation!

Unlock Your Light: Join Lightworkers Worldwide on CrystalWind.ca!

 

 

Follow Us!

 

 

Who is Online Now

We have 29921 guests and no members online

Featured This Month

Page:

Bright Beltane Blessings!

Bright Beltane Blessings!

The wheel turns to Beltane, also known as Mayday, marking the beginning of S... Read more

Birth Totem - Falcon

Birth Totem - Falcon

Birth Totem Falcon Birth dates: March 21 – April 19 Birth Totem is: Falcon... Read more

Aries Mythology

Aries Mythology

The Mythology of Aries First things first, when referring to the mythology ... Read more

Sun in Aries

Sun in Aries

Aries March 21 through April 20 An Overview of Sun Sign Characteristics for A... Read more

Beltane

Beltane

Beltane Ritual Celebrated May 1st Beltane is also known as May Day, Walpurg... Read more

Dandelion

Dandelion

Reminds you of the abundance life holds. Gender: Masculine Planet: Jupiter E... Read more

Aries Spiritual Stone - Eudialyte

Aries Spiritual Stone - Eudialyte

Eudialyte Birthstone: Aries Planet: Mars Element: Fire Chakra: Heart E... Read more

Green Aventurine

Green Aventurine

The Emotional Balancer Stone Green Aventurine is perhaps the best balancing... Read more

Diamond

Diamond

The King of all Stones The diamond symbolizes wisdom and enlightenment, pur... Read more

The Time of No Time: Beltane!

The Time of No Time: Beltane!

Around the medicine wheel of life we go, from season to season (solstice to ... Read more

Budding Trees Moon

Budding Trees Moon

Red Hawk - Fire Opal - Dandelion - Yellow March 21 – April 19 The Budding Tr... Read more

The Crystal Wind Oracle Card Deck

The Crystal Wind Oracle Card Deck

The Crystal Wind Oracle™ The Crystal Wind Oracle Myth & Magic Card D... Read more

Hematite

Hematite

The Grounding Stone With its iron content, hematite has a strengthening inf... Read more

Aries

Aries

ARIES Mar 21 - Apr 20 Spirit: Adventurous, courageous Objectives: To plan... Read more

Bloodstone

Bloodstone

The Blood Energizer Stone The combined colours of bloodstone allow it to fu... Read more

© 2008-2024 CrystalWind.ca. All rights reserved. Site Creation by CrystalWind.ca.
Web Hosting by Knownhost.com

 

 

X

Right Click

No right click